Plasmid

Part:BBa_K3788022:Design

Designed by: Rebecca Pagès   Group: iGEM21_Aix-Marseille   (2021-09-28)


pRL1 (caa:gfp cai cal) Version 2


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 1568
    Illegal PstI site found at 1671
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 2266
    Illegal PstI site found at 1568
    Illegal PstI site found at 1671
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 2586
    Illegal BamHI site found at 2259
    Illegal XhoI site found at 2135
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 1568
    Illegal PstI site found at 1671
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 1568
    Illegal PstI site found at 1671
    Illegal NgoMIV site found at 2335
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 867
    Illegal SapI.rc site found at 2089


Design Notes

-


Source

Sequences were given by LISM laboratory from CNRS. GFP coding sequence was taken corresponding to Part:BBa_E0040


References